|
| Status |
Public on Apr 09, 2014 |
| Title |
A2EN cells_Chlamydia G1V_4h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Human A2EN_Chlamydia G1V_4 hrs
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A2EN
cell type: Human endocervical epithelial cells
infected with: Chlamydia G1V mutant
time point: 4 hr post infection
|
| Treatment protocol |
A2EN cells were infected with different strains of Chlamydia trachomatis at an MOI of 10 by spinning at 3000 rpm for 30 min at 10 degree and shift to 37 degree incubator for 4 hours.
|
| Growth protocol |
Human endocervical epithelial A2EN cells were maintained in keratinocyte-SFM medium supplemented with 10 % FBS, 0.5 ng/mL human recombinant epidermal growth factor and 50 μg/mL bovine pituitary extract.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Samples were collected using QIAGEN RNeasy Plus Mini Kit as described by the manufacturer.
|
| Label |
Biotin-UTP
|
| Label protocol |
Hybridization targets were prepared with MessageAmp Premier RNA Amplification Kit from total RNA
|
| |
|
| Hybridization protocol |
Hybridization was achieved in Affymetrix GeneChip hybridization oven 645 according to standard Affymetrix GeneChip protocol
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 7G according to standard Affymetrix GeneChip protocol
|
| Description |
A2ENG1VR2
|
| Data processing |
The data were analyzed with Partek Genomic Suite 6.6 using Affymetrix default analysis settings, quantile normalization and RMA background correction.
|
| |
|
| Submission date |
Jan 23, 2014 |
| Last update date |
Apr 09, 2014 |
| Contact name |
Raphael H Valdivia |
| E-mail(s) |
[email protected]
|
| Phone |
9196683831
|
| Organization name |
Duke University
|
| Department |
Molecular Genetics and Microbiology
|
| Lab |
Valdivia
|
| Street address |
207 Research drive, Jones bldg 272
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE54336 |
Expression data of A2EN cells during early stage of Chlamydia trachomatis infection |
|
