|
| Status |
Public on Jan 23, 2014 |
| Title |
NCI-H1666 MK2206 treated-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Human non-small cell lung cancer line NCI-H1666
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: non-small cell lung cancer
cell line: NCI-H1666
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using RNAeasy plus mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Illumina TotalPrep 96 RNA Amplification Kit was used to amplify and label the RNA
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
NCI-H1666 mock-infected MK2206-treated
|
| Data processing |
The data were processed using Limma and Beadarray R packages from Bioconductor suite. The raw data was exported from GenomeStudio and log2 transformed prior to quantile normalization. We applied IlluminaHumanv3.db annotation data freely available from Bioconductor in order to map Illumina probes to known genes and annotate the probes. To rank the genes in order of evidence for differential expression we computed moderated t-statistics and log-odds of differential expression by Empirical Bayes estimator.
|
| |
|
| Submission date |
Jan 22, 2014 |
| Last update date |
Jan 23, 2014 |
| Contact name |
Denis Kainov |
| E-mail(s) |
[email protected]
|
| Organization name |
Helsinki University
|
| Department |
Institute for Molecular Medicine Finland
|
| Lab |
Medical systems virology
|
| Street address |
Tukholmankatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
00290 |
| Country |
Finland |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE54293 |
Akt inhibitor MK2206 prevents influenza A(H1N1)pdm09 virus infection in vitro |
|
