|
| Status |
Public on Mar 06, 2014 |
| Title |
B6 retinal explant cultured for three days with chir99021, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
whole retinal tissue from B6 mouse cultured for three days with chir99021 (15uM)
|
| Organism |
Mus musculus |
| Characteristics |
tissue: neural retina
protocol: retinal explant cultured for three days with chir99021
strain: C57BL/6NCrSlc
age: 10 weeks old
|
| Treatment protocol |
Eyes were enucleated from mice, and the neural retina was separated from the cornea, the sclera, the lens, the iris, the ciliary body, and the pigmented epithelium
|
| Growth protocol |
mice were maintained under a 12 h light/dark cycle with access to food and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy (QIAGEN) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA, according to the 3' IVT Expression Kit User Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430.2.0 Array.
|
| Scan protocol |
GeneChips were scanned using the Scanner 3000 7G
|
| Description |
Gene expression data from damaged retina supplemented with GSK3 inhibitor, B6
11062106_B6chiron2_MG430_2
|
| Data processing |
The data were analyzed with RMA algorithm in the R/Bioconductor (http://www.bioconductor.org/) package
|
| |
|
| Submission date |
Jan 14, 2014 |
| Last update date |
Mar 06, 2014 |
| Contact name |
Masayo Takahashi |
| Organization name |
RIKEN CDB
|
| Lab |
Laboratory for Retinal Regeneration
|
| Street address |
2-2-3 Minamimachi, Minatojima, Chu-o-ku
|
| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE54056 |
Expression data from adult mouse normal and damaged retina from B6 and 129 mouse strains |
|
