NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1303101 Query DataSets for GSM1303101
Status Public on Aug 07, 2014
Title BF638R DM-mucin media rep1
Sample type RNA
 
Source name grown in vitro to mid-log phase in defined media with mucin glycans
Organism Bacteroides fragilis 638R
Characteristics treatment: grown in vitro to mid-log phase in defined media with mucin glycans
sample type: grown in vitro to mid-log phase in defined media with mucin glycans
Growth protocol For in vitro samples: Overnight cultures of Bacteroides fragilis 638R were diluted into defined media contain either 0.5% glucose or 2% mucin glycans, and grown to mid-logarithmic phase at A550 of 0.5 complex media ( Rocha et al. 1996, J. Bacteriol. 178:6895). For in vivo samples, BF638R cells were grown in a tissue cage in rats and sampled on days 1,4, and 8 post-inoculation as described by Lobo et al. 2013, MicrobiologyOpen 2:326.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated by the hot phenol method and treated as described by Sund et al, 2008, Mol. Microbiol.67:129-142.
Label Cy3
Label protocol Labeling was performed by at the FSU NimbleGen Microarray Facility, using standard protocols as described previously (Sund et al., 2008, Mol. Microbiol. 67:129-142).
 
Hybridization protocol Hybridization was performed by at the FSU NimbleGen Microarray Facility, using standard protocols as described previously (Sund et al., 2008, Mol. Microbiol. 67:129-142)
Scan protocol Scanning was performed by at the FSU NimbleGen Microarray Facility, using standard protocols as described previously (Sund et al., 2008, Mol. Microbiol. 67:129-142)
Data processing Raw data (pair files) were subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185) using NimbleGen Systems DEVA 1.1 software as described on their web site. Raw data (pair files) were subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185) using DEVA 1.1 software downloaded from Roche/NimbleGen as described on their web site (http://www.nimblegen.com/downloads/support/06271090001_DEVA_UserGuide_v1p2.pdf).
 
Submission date Jan 07, 2014
Last update date Aug 07, 2014
Contact name Charles Jeffrey Smith
E-mail(s) [email protected]
Phone 252-744-2700
Fax 252-744-3104
Organization name East Carolina University
Department Microbiology & Immunology
Street address 600 Moye Blvd.
City Greenville
State/province NC
ZIP/Postal code 27834
Country USA
 
Platform ID GPL15788
Series (2)
GSE53883 Bacteroides fragilis in vivo gene expression
GSE73107 Cis-encoded sRNAs repress expression of polysaccharide utilization loci in Bacteroides fragilis 638R

Data table header descriptions
ID_REF
VALUE The RMA processed data was averaged and log2 transformed (see *.calls files)

Data table
ID_REF VALUE
BF638000000000001 9.54421
BF638000000000002 5.70756
BF638000000000003 5.43141
BF638000000000004 7.19421
BF638000000000005 9.7504
BF638000000000006 11.1139
BF638000000000007 8.44971
BF638000000000008 6.43665
BF638000000000009 7.13119
BF638000000000010 6.88373
BF638000000000011 5.26056
BF638000000000012 8.1583
BF638000000000013 7.08379
BF638000000000014 11.07084
BF638000000000015 11.09903
BF638000000000016 10.51889
BF638000000000017 10.0243
BF638000000000018 11.74744
BF638000000000019 11.60772
BF638000000000020 12.69021

Total number of rows: 4341

Table truncated, full table size 111 Kbytes.




Supplementary file Size Download File type/resource
GSM1303101_DM-mucin_rep1.calls.gz 284.8 Kb (ftp)(http) CALLS
GSM1303101_DM-mucin_rep1.pair.gz 5.8 Mb (ftp)(http) PAIR
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap