|
| Status |
Public on Jun 09, 2015 |
| Title |
genomic DNA from whole blood of HIV+ man 2 [2012] |
| Sample type |
genomic |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
tissue: whole blood
dnamage: 62.7561269
chipid: 8221916133
hivstatus: positive
age (yrs): 61
bmi: 23
cd4low: 383
hiv.viral.load: 10
|
| Treatment protocol |
Standard Illumina Protocol
|
| Growth protocol |
Fresh frozen blood
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted and purified from fresh frozen tissue using Qiagen DNeasy Kit according to standard instructions
|
| Label |
Cy3 and Cy5
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
Sample 2
|
| Data processing |
BeadStudio software v3.2. The non_normalized.txt contains unmethylated and methylated signal intensities.
|
| |
|
| Submission date |
Jan 06, 2014 |
| Last update date |
Jun 09, 2015 |
| Contact name |
Steve Horvath |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California, Los Angeles
|
| Department |
Human Genetics
|
| Lab |
Horvath
|
| Street address |
695 Charles E. Young Drive South, Box 708822
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7088 |
| Country |
USA |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE53841 |
Whole blood DNA methylation samples from HIV positive men [2012 study] |
|
