|
| Status |
Public on May 25, 2014 |
| Title |
ALK5Ctrl mouse3, rep.1 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampus
|
| Organism |
Mus musculus |
| Characteristics |
background strain: FVB/N
age: 3 months
tissue: Hippocampus
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Nanodrop Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were processed with Illumina's BeadStudio and Significance Analysis of Microarrays (SAM) 3.0
Normalized values are simple median centering of the arrays (processsed by SAM)
|
| |
|
| Submission date |
Jan 02, 2014 |
| Last update date |
May 25, 2014 |
| Contact name |
Yingbo He |
| E-mail(s) |
[email protected]
|
| Organization name |
Stanford University
|
| Street address |
Stanford University
|
| City |
Stanford |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE53761 |
Analysis of gene expression in ALK5Ctrl and ALK5CA mouse hippocampus |
|
