|
| Status |
Public on May 15, 2014 |
| Title |
DK-first |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs from CIS patient before IFNb-1a in-vivo treatment
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: PBMCs
treatment: before IFNb-1a in-vivo treatment
|
| Treatment protocol |
6x107 PBMCs were separated from 6 CIS patients at baseline and after 12 months of IFNb-1a (44 ug s.q. tiw) treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a Rneasy kit according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7 ug total RNA.
|
| |
|
| Hybridization protocol |
15 ug of cRNA was used for gene array hybridization. Arrays were hybridized for 16 hours at 45oC in the GeneChip® Hybridization Oven 640.The arrays were washed and stained with R-phycoerythrin streptavidin in the GeneChip® Fluidics Station 400.
|
| Scan protocol |
The arrays were scanned with the Hewlett Packard GeneArray Scanner.
|
| Data processing |
Affymetrix GeneChip® Microarray Suite 5.0 software
|
| |
|
| Submission date |
Dec 30, 2013 |
| Last update date |
Jul 21, 2014 |
| Contact name |
Silva Markovic-Plese |
| E-mail(s) |
[email protected]
|
| Phone |
919 966 3701
|
| Organization name |
University of North Carolina at Chapel Hill
|
| Street address |
105 Mason Farm Rd
|
| City |
Chapel Hill |
| State/province |
NC |
| ZIP/Postal code |
27516 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE53716 |
IFNb-1a in-vivo treatment induces the expression and signaling of IFNAR1 and inhibits Th17 responses in PBMCs derived from CIS patients |
|
