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Sample GSM1294374 Query DataSets for GSM1294374
Status Public on Dec 19, 2013
Title Tg4510 control mouse rep1
Sample type RNA
 
Source name P0 injected mouse analyzed at 4 months of age
Organism Mus musculus
Characteristics strain: Tg4510
treatment: control mouse
tissue: hippocampus
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 250 ng total RNA (Affymetrix 3'IVT Express Manual, 2008-2010, RevB, Affymetrix).
 
Hybridization protocol Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C, 60 RPM on GeneChip Mouse Expression 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 and the Affymetrix Command Console v3.0 (AGCC) software.
Description Gene expression data from brain
Data processing The data were analyzed with Expression Console using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Dec 18, 2013
Last update date Dec 19, 2013
Contact name Horatiu Voicu
Organization name Baylor College of Medicine
Street address One Baylor Plaza
City Houston
ZIP/Postal code 77030
Country USA
 
Platform ID GPL1261
Series (1)
GSE53480 Expression data from Tg4510 and Wild-type mice after AAVTFEB injection

Data table header descriptions
ID_REF
VALUE log2 GC-RMA signal

Data table
ID_REF VALUE
1415670_at 9.194974
1415671_at 12.35802
1415672_at 11.92232
1415673_at 9.033848
1415674_a_at 9.676755
1415675_at 9.631336
1415676_a_at 11.53558
1415677_at 10.68704
1415678_at 10.8715
1415679_at 11.06652
1415680_at 8.275179
1415681_at 9.974021
1415682_at 8.552065
1415683_at 11.18358
1415684_at 8.810971
1415685_at 9.494419
1415686_at 11.06298
1415687_a_at 13.12595
1415688_at 10.64556
1415689_s_at 9.179576

Total number of rows: 45101

Table truncated, full table size 894 Kbytes.




Supplementary file Size Download File type/resource
GSM1294374_irs_ZE_8284_MA2_32022.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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