|
| Status |
Public on Dec 08, 2014 |
| Title |
TC1A9_IFN_2 |
| Sample type |
RNA |
| |
|
| Source name |
TC1A9_IFN
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
cell line: TC-1/A9
cell type: tumour cells cultured in vitro.
treated with: 50 U/ml IFNγ
|
| Treatment protocol |
Cells were cultured in fresh medium for 24 h, after which the medium was removed and the cells were grown in medium containing IFNγ (50 U/ml, R&D Systems, Minneapolis, USA ) for additional 24h.
|
| Growth protocol |
TC-1/A9 cells were maintained in RPMI 1640 medium supplemented with 10% FCS, 2 mM L-glutamine and antibiotics, cultured at 37°C in a humidified atmosphere with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy Mini Kit Quiagen
|
| Label |
biotin (Cy3)
|
| Label protocol |
Standard Illumina labeling protocol (TotalPrep RNA Amplification Kit)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol (6-Sample BeadChip with IntelliHyb Seal)
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
TC1A9_IFN_2
|
| Data processing |
Quantile normalisation (limma package), Log2 transformation. Probes were filtered to be present (detection p-value<0,01) in at least one sample when creating comparisons.
|
| |
|
| Submission date |
Dec 18, 2013 |
| Last update date |
Dec 08, 2014 |
| Contact name |
Milan Reinis |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Molecular Genetics of the ASCR, v. v. i.
|
| Lab |
Laboratory of Tumour Immunology
|
| Street address |
Videnska 1083
|
| City |
Prague |
| ZIP/Postal code |
14220 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL17543 |
| Series (2) |
| GSE53467 |
Epigenetic regulations in the IFNγ signalling pathway: IFNγ-mediated MHC class I upregulation on tumour cells is associated with DNA demethylation of antigen-presenting machinery genes [TC1A9_IFN] |
| GSE53469 |
Epigenetic regulations in the IFNγ signalling pathway: IFNγ-mediated MHC class I upregulation on tumour cells is associated with DNA demethylation of antigen-presenting machinery genes |
|
