|
| Status |
Public on Dec 07, 2013 |
| Title |
Normal thyroid, fresh-frozen, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
fresh-frozen normal thyroid tissue from contralateral lobe of thyroid tumor
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age at diagnosis (yrs): 34
tissue type: normal thyroid
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted and purified using the RNeasy Mini kit (Quiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA
|
| |
|
| Hybridization protocol |
Labelling, hybridization and scanning protocols were done in a certified Affymetrix Core Facility at Instituto Gulbenkian de Ciência (Oeiras, Portugal)
|
| Scan protocol |
Labelling, hybridization and scanning protocols were done in a certified Affymetrix Core Facility at Instituto Gulbenkian de Ciência (Oeiras, Portugal)
|
| Description |
Hs_A506_Normal
|
| Data processing |
The data were analyzed with DNA-Chip Analyzer (dChip) 2010.01 software. Arrays were normalized with the invariant set normalization method and gene-level expression was determined by summarizing the multiple probes across the gene (median of 26 probes per gene) into a single gene level-probe set expression, using model-based expression analysis with perfect match-only model.
|
| |
|
| Submission date |
Dec 06, 2013 |
| Last update date |
Dec 07, 2013 |
| Contact name |
Branca Maria Cavaco |
| E-mail(s) |
[email protected]
|
| Organization name |
Instituto Português de Oncologia de Lisboa Francisco Gentil (IPOLFG)
|
| Department |
Unidade de Investigação de Patobiologia Molecular (UIPM)
|
| Street address |
Rua Prof. Lima Basto
|
| City |
Lisboa |
| ZIP/Postal code |
1099-023 |
| Country |
Portugal |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE53072 |
Gene expression profilling of anaplastic thyroid carcinomas (ATC) and normal thyroid tissues |
|
