|
| Status |
Public on Nov 30, 2013 |
| Title |
HSCs from male mice treated with E2, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
HSCs from male mice treated with E2, replicate 3
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
agent: E2
cell type: Hematopoietic stem cells
|
| Treatment protocol |
Mice were either treated with control corn oil (100 micro L) or 2 micro gram estradiol in corn oil (100 micro L) subcutaneously.
|
| Growth protocol |
Young adult mice (2-3 month old) fed ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
5000-7000 HSCs (CD150+ CD48- c-kit+ Sca-1+ lineage- cells) were sorted directly into Trizol reagent by flow cytometry.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared using Nugen Ovation Pico WTA V2 system and the Encore Biotin Module (NuGEN Technologies, San Carlos, CA)
|
| |
|
| Hybridization protocol |
2.5µg of labeled cDNA were hybridized to Affymetrix Mouse Gene ST 1.0 microarrays.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix’s GeneChip® Scanner 3000 and the GeneChip Command Console software v3.0
|
| Description |
Gene expression data from HSCs isolated from male mice treated with E2
ME3 HSC
|
| Data processing |
The data were analyzed with dChip software using default analysis settings.
|
| |
|
| Submission date |
Nov 25, 2013 |
| Last update date |
Nov 30, 2013 |
| Contact name |
Daisuke Nakada |
| E-mail(s) |
[email protected]
|
| Organization name |
Baylor College of Medicine
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE52711 |
Expression data from murine hematopoietic stem cells isolated from mice treated with control oil or estradiol |
|
