|
| Status |
Public on Oct 06, 2014 |
| Title |
SMC_24h_H1 |
| Sample type |
RNA |
| |
|
| Source name |
pBSMCs_PDGF 4hr
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bladder
cell type: Human primary bladder smooth muscle cells (pBSMCs)
treated with: 1 nM PDGF-BB for 4hrs
|
| Treatment protocol |
pBSMCs were serum starved overnight and treated with 1 nM PDGF for 0, 4, and 24 h.
|
| Growth protocol |
Human primary bladder smooth muscle cells (pBSMCs) were cultured in smooth muscle cell medium (SMCM, Sciencell Research Laboratories, Carlsbad, CA) at 37°C in a humidified incubator with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from pBSMCs was extracted using the TRIzol reagent followed by RNeasy (QIAgen) method. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Total RNA was labeled employing the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s instructions (Affymetrix)
|
| |
|
| Hybridization protocol |
The samples were hybridized to HuGene ST1.0 microarrays according to standardized protocols from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip® Scanner 3000 7G (Affymetrix)
|
| Description |
SAMPLE 5
|
| Data processing |
The microarray data were preprocessed in Expression Consol software (Affymetrix) employing the probe logarithmic intensity error algorithm (PLIER, parameters - PM-GCBG background subtraction, quantile normalization, iterPLIER summarization)
|
| |
|
| Submission date |
Nov 19, 2013 |
| Last update date |
Oct 06, 2014 |
| Contact name |
Sungyong You |
| E-mail(s) |
[email protected]
|
| Organization name |
Cedars-Sinai Medical Center
|
| Department |
Surgery
|
| Lab |
Freeman Lab.
|
| Street address |
Beverly Blvd 8400
|
| City |
LA |
| State/province |
CA |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE52488 |
Gene expression profiling regulated by PDGF in pBSMCs |
|
