|
| Status |
Public on Nov 19, 2013 |
| Title |
primary_lung_cancer_B086 |
| Sample type |
RNA |
| |
|
| Source name |
primary lung cancer
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: primary lung cancer
Post Surgical Treatment: ACT
Stage: 2B
age: 71
Sex: Male
Cause of death: Lung cancer
Histology type: ADC
OS time: 0.97
OS status: Dead
dss survival time: 0.97
DSS status: Dead
dcc sample: Yes
predominant subtype: Micropapillary
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
The data were preprocessed using RMA v0.5.
|
| |
|
| Submission date |
Nov 18, 2013 |
| Last update date |
Dec 29, 2014 |
| Contact name |
Ming Tsao |
| E-mail(s) |
[email protected]
|
| Phone |
4169464501
|
| Organization name |
University of Toronto
|
| Department |
Ontario Cancer Institute
|
| Lab |
Applied Molecular Oncology
|
| Street address |
610 University Avenue
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G2M9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE14814 |
Prognostic and Predictive Gene Signature for Adjuvant Chemotherapy in Resected Non-Small-Cell Lung Cancer |
|
