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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 10, 2006 |
| Title |
liver_ABCA1-TG, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
liver, ABCA1-TG
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender:male
Tissue:liver
|
| Treatment protocol |
Diet: NIH36, livers were snap-frozen and stored at -80C
|
| Growth protocol |
Male mice were sacrificed at 5 to 6 months old
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| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction, Qiagen RNEasy clean-up with on-column DNAse treatment
|
| Label |
biotin
|
| Label protocol |
Samples prepared with Arcturus RiboAmp protocol from 2 ug of total RNA
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| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of cRNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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| Scan protocol |
GeneChips were scanned using the Affymetrix (HP) GeneArray Scanner G2500A.
|
| Description |
Gene expression data comparing ABCA1 over-expression in LDL receptor +/- genetic background
|
| Data processing |
RMA-normalization
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| |
|
| Submission date |
Aug 10, 2006 |
| Last update date |
Mar 27, 2007 |
| Contact name |
Eric Billings |
| Organization name |
NHLBI
|
| Street address |
10 Center Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20874 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE5496 |
Gene expression series from murine liver samples comparing ABCA1 over-expression in LDL receptor -/- genetic background |
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| Data table header descriptions |
| ID_REF |
|
| VALUE |
RMA-normalized Signal intensity |
| ABS_CALL |
MAS 5.1 call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC) |
| DETECTION P-VALUE |
|
| Data table |
| ID_REF |
VALUE |
ABS_CALL |
DETECTION P-VALUE |
| AFFX-BioB-5_at |
909.4 |
P |
0.000297 |
| AFFX-BioB-M_at |
1746.7 |
P |
0.000127 |
| AFFX-BioB-3_at |
723.6 |
P |
0.000044 |
| AFFX-BioC-5_at |
2134.9 |
P |
0.00007 |
| AFFX-BioC-3_at |
1357.1 |
P |
0.000052 |
| AFFX-BioDn-5_at |
1474.8 |
P |
0.000044 |
| AFFX-BioDn-3_at |
8508.7 |
P |
0.000052 |
| AFFX-CreX-5_at |
19872.1 |
P |
0.000044 |
| AFFX-CreX-3_at |
24984.2 |
P |
0.000044 |
| AFFX-DapX-5_at |
39.7 |
A |
0.185131 |
| AFFX-DapX-M_at |
55.5 |
A |
0.131361 |
| AFFX-DapX-3_at |
6.9 |
A |
0.916426 |
| AFFX-LysX-5_at |
27.9 |
A |
0.239063 |
| AFFX-LysX-M_at |
68.9 |
A |
0.48511 |
| AFFX-LysX-3_at |
39.1 |
A |
0.327079 |
| AFFX-PheX-5_at |
4.2 |
A |
0.960339 |
| AFFX-PheX-M_at |
1.5 |
A |
0.949771 |
| AFFX-PheX-3_at |
26.2 |
A |
0.52976 |
| AFFX-ThrX-5_at |
4.5 |
A |
0.860518 |
| AFFX-ThrX-M_at |
43.3 |
A |
0.455413 |
Total number of rows: 22690
Table truncated, full table size 624 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM126581.CEL.gz |
3.4 Mb |
(ftp)(http) |
CEL |
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