|
| Status |
Public on Mar 07, 2016 |
| Title |
D1_CD4+CD3/CD28+TNF |
| Sample type |
RNA |
| |
|
| Source name |
CD4+CD3/CD28+TNF
|
| Organism |
Homo sapiens |
| Characteristics |
donor id: Donor 1
tissue: Peripheral blood
cell type: CD4+ T cells
stimulation i: CD3/CD28/MHC-I coated beads
stimulation ii: TNF
|
| Treatment protocol |
After extensive washing, cells were lysed in Trizol reagent
|
| Growth protocol |
CD4+ T cells were isolated using CD4+ RosetteSep and cultured for 3 days in medium with or without CD3/CD28/MHC-I-coated beads and TNF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the TargetAmp Nano-g Biotin-aRNA Labeling Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4958478054_L
|
| Data processing |
The data were normalised using quantile normalisation in Partek Genomic Suite 6.6 (v.6.12.1227)
|
| |
|
| Submission date |
Nov 07, 2013 |
| Last update date |
Mar 07, 2016 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
[email protected]
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE52182 |
TNF dependent induction of inhibitory pathways in CD4+ T cells in HIV and LCMV [TNF stimulation] |
| GSE52185 |
TNF dependent induction of inhibitory pathways in CD4+ T cells in HIV and LCMV |
|
