|
| Status |
Public on Oct 16, 2006 |
| Title |
S. cerevisiae dbr1delta, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Log phase yeast culture
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Diploid yeast strain; Genotype MATa/MATalpha his3?delta1/his3delta1 leu2delta0/leu2?delta0 lys2delta0/+ met15delta0/+ ura3delta0/ura3delta0 dbr1
|
| Biomaterial provider |
Z. Zhang, University of Washington
|
| Treatment protocol |
Log phase yeast cultures harvested by centrifugation
|
| Growth protocol |
Yeast were grown in a shaking flask to log phase in YPD at 30 C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using phenol extraction, treated with DNase and purified using a Qiagne RNeasy kit.
|
| Label |
Double-stranded cDNA was created using the Affymetrix WT Double-Stranded cDNA Synthesis Kit, and cDNA probes were labeled using the Affymetrix WT Double-Stranded DNA Terminal Labeling Kit.
|
| Label protocol |
Samples were labeled according to the manufacturer's instructions.
|
| |
|
| Description |
Gene expression data from log phase BY4743 yeast cells
|
| Data processing |
Data was processed using Affymetrix Tiling Array Software. Data was quantile normalized, and log2 signals from each probe were reported.
|
| |
|
| Submission date |
Aug 07, 2006 |
| Last update date |
Oct 16, 2006 |
| Contact name |
Jay R. Hesselberth |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Colorado School of Medicine
|
| Department |
Biochemistry and Molecular Gentetics
|
| Lab |
Jay Hesselberth
|
| Street address |
12801 E 17TH AVE
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL4065 |
| Series (1) |
| GSE5470 |
Expression data from BY4743 and dbr1 yeast |
|
