|
| Status |
Public on Sep 24, 2014 |
| Title |
control3 |
| Sample type |
RNA |
| |
|
| Source name |
murine splenic b cells control
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
cell type: splenic B-cells
transduction: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Mini-Kit from QIAGEN according to the manufacturer's recommendations.
|
| Label |
biotin
|
| Label protocol |
Labeling was done according to the manufacturer's recommendations (Illumina).
|
| |
|
| Hybridization protocol |
Hybridization was done according to the manufacturer's recommendations (Illumina).
|
| Scan protocol |
Arrays were scanned on a Bead Array Reader according to the manufacturers recommendations (Illumina).
|
| Description |
control
|
| Data processing |
Background correction was done according to Illuminas proprietary correction algorithm, and data were normalized using quantile normalization. All processing was done using R package beadarray, which does not give any detection p values.
|
| |
|
| Submission date |
Oct 25, 2013 |
| Last update date |
Sep 24, 2014 |
| Contact name |
Karl Koechert |
| E-mail(s) |
[email protected]
|
| Organization name |
Max-Delbrueck-Centre for Molecular Medicine
|
| Street address |
Robert-Roessle-Str. 10
|
| City |
Berlin |
| ZIP/Postal code |
13125 |
| Country |
Germany |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE51719 |
Expression analysis of murine splenic B-cells after retroviral transduction with a constitutively active variant of IRF5 (IRF5-4D) |
|
