|
| Status |
Public on Aug 08, 2006 |
| Title |
Double_positive_thymocytes_Dexamethasone (1E-07 M) _3h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
CD4+CD8+ double positive (DP) thymocytes 3h Dexamethasone(DEX) (final concentration 1E-07 M) treated
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C3H/HeN
Gender: female
Age: 4 weeks
Tissue: thymus
|
| Treatment protocol |
CD4+CD8+ double positive thymocytes were treated with medium and Dexamethasone (final concentration 1E-07 M) in PBS for 3 hours
|
| Growth protocol |
CD4+CD8+ double positive thymocytes were plated in 6 well plates with 3 ml of the culture medium at a concentration of 5.0E+06 cell/ml and kept at 37°C , 5% CO2 in a humidified atmosphere for 3 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with TRIzol LS reagent(Invitrogen)
|
| Label |
biotin
|
| |
|
| Description |
Thymi from 4 week old C3H/HeN mice were teased in culture medium, CD4+CD8+ double positive (DP) thymocytes were sorted with microbeads Miltenyi Biotech methods and were plated in 6 well plates with 3 ml of the culture medium at a concentration of 5.0E+06 cell/ml together with 1E-07 M Dexamethasone or a corresponding volume of PBS. DP thymocytes were kept at 37°C, 5% CO2 in a humidified atmosphere for 3 hours
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Aug 04, 2006 |
| Last update date |
Aug 07, 2006 |
| Contact name |
Carlo Riccardi |
| E-mail(s) |
[email protected]
|
| Phone |
+390755857467
|
| Fax |
+390755857405
|
| Organization name |
Università degli Studi di Perugia
|
| Department |
Clinical and Experimetal Medicine
|
| Lab |
Pharmacology, Toxicology, Chemotherapy
|
| Street address |
via del Giochetto
|
| City |
Perugia |
| State/province |
Perugia |
| ZIP/Postal code |
06100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5463 |
Modulation of pro- and antiapoptotic molecules in double-positive (CD4+CD8+) thymocytes following dexamethasone treatmen |
|
