|
| Status |
Public on Jun 09, 2014 |
| Title |
Klotho-treated MO3.13 human oligodendroglioma cell line Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Klotho-treated MO3.13 human oligodendroglioma cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MO3.13
cell type: oligodendroglioma cell line
treatment: Klotho-treated
|
| Growth protocol |
MO3.13 cells were grown in DMEM containing 10 % fetal bovine serum (FBS), 50 U/ml penicillin, 50 mg/ml streptomycin, and 1 mM of L-glutamine andSodium pyruvate. Cells were treated with 0.4 ug/mL of recombinant mouse Klotho in serum free DMEM for 3.5 hours and total RNA was collected for microarray
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol isolation followed by Rneasy (Qiagen) column purification were performed according to manufacturer's specifications
|
| Label |
biotin
|
| Label protocol |
Preparation of biotinylated cDNA was performed according to the standard Nugen amplification sample preparation protocol, starting with 100ng of total RNA
|
| |
|
| Hybridization protocol |
cDNA was fragmented and 10ug hybridized to an Affymetrix Human Exon 1.0 ST Array for 16 hours. Arrays were washed and stained using the Affy 450 Fluidics Station according to the manufacturer's protocol
|
| Scan protocol |
Arrays were scanned using a GeneChip 3000 scanner according to the manufacturers recommendation
|
| Description |
Gene expression data obtained from MO3.13 human oligodendroglioma cell line
|
| Data processing |
RMA
|
| |
|
| Submission date |
Oct 22, 2013 |
| Last update date |
Jun 09, 2014 |
| Contact name |
Hu Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Mayo Clinic
|
| Department |
Molecular Pharmacology & Experimental Therapeutics
|
| Lab |
Systems Biology and Pharmacology
|
| Street address |
200 First Street, Gonda Building G19-408
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55904 |
| Country |
USA |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE51536 |
The Anti-Aging and Tumor Suppressor Protein Klotho Affects Signaling Pathwaysin a Human Oligodendroglioma Cell Line |
|
