|
| Status |
Public on May 19, 2014 |
| Title |
Mouse E18.5 left ventricle tissue E18L.2 |
| Sample type |
RNA |
| |
|
| Source name |
Left ventricle tissues from mouse embryo at E18.5
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Left ventricle tissues
age: E18.5
strain: CD1
|
| Treatment protocol |
For the first developmental stage, embryonic day (E) 7.5, whole embryos were collected. For later stages cardiac structures were micro-dissected including E8.5 heart tubes and left and right ventricles corresponding to E9.5, E12.5, E14.5, E18.5, newborn and adult mice. All samples were harvested in biological triplicates, with approximately 30-50 embryos/hearts included per replicate corresponding to E7.5, E8.5 and E9.5; 10-20 hearts for each E12.5 or E14.5 sample; 3-5 hearts pooled for each E18.5 replicate and one used for newborn and adult samples. Embryonic stem cells (ESC) from the R1 line were used as pluripotent gold standard. To avoid interference due to high protein content newborn and adult hearts were fast-frozen and pulverized prior to RNA extraction.
|
| Growth protocol |
Wild type CD1 strain mouse embryos were harvested at defined stages from timed mating pairs. Morphology and somite count were used as inclusion criteria for each developmental time point.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Left ventricle tissues from mouse embryo at E18.5
|
| Data processing |
The data were analyzed with R/Bioconductor using RMA.
|
| |
|
| Submission date |
Oct 21, 2013 |
| Last update date |
May 19, 2014 |
| Contact name |
XING LI |
| E-mail(s) |
[email protected]
|
| Organization name |
Mayo Clinic
|
| Department |
Health Sciences Research
|
| Street address |
200 1st street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE51483 |
Transcriptional Atlas of Cardiogenesis Maps Congenital Heart Disease Interactome |
|
