|
| Status |
Public on Aug 07, 2006 |
| Title |
hippocampus_129S1SvImJ_3_120902 |
| Sample type |
RNA |
| |
|
| Source name |
Hippocampal total RNA
|
| Organism |
Mus musculus |
| Characteristics |
strain (129S1/SvImJ)
gender (male)
age (13-14 weeks)
tissue (hippocampus)
|
| Biomaterial provider |
Biomaterial provider Jackson Laboratories (Bar Harbor, ME, USA)
|
| Treatment protocol |
No treatment was given as the study was an inbred strain comparision.
|
| Growth protocol |
Every effort was made to keep the environment constant for all study mice. Mice were killed by cervical dislocation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Guanidium thiocyanate method of Chomczynski and Sacchi (Anal Biochem, 1987, 162:156-159) using Trizol reagent.
|
| Label |
Streptavidin
|
| Label protocol |
Using standard Affymetrix protocols (Affymetrix 1999 Genechip Expression Technical Manual, Affymetrix Santa Clara USA)
|
| |
|
| Hybridization protocol |
Using standard Affymetrix protocols (Affymetrix 1999 Genechip Expression Technical Manual, Affymetrix Santa Clara USA)
|
| Scan protocol |
Using standard Affymetrix protocols (Affymetrix 1999 Genechip Expression Technical Manual, Affymetrix Santa Clara USA)
|
| Description |
For full description of the experiment, refer to Fernandes et al (2004) Eur J Neuroscience 19:2576-2582
|
| Data processing |
Using Affymetrix global scaling (TGT 100) and MAS5 algorithm
|
| |
|
| Submission date |
Aug 01, 2006 |
| Last update date |
Aug 02, 2006 |
| Contact name |
Cathy Fernandes |
| E-mail(s) |
[email protected]
|
| Organization name |
Rudolf Magnus Institute of Neuroscience
|
| Department |
Pharmacology & Anatomy
|
| Lab |
Behavioural Genomics
|
| Street address |
Universiteitsweg 100
|
| City |
Utrecht |
| ZIP/Postal code |
3584 CG |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE5429 |
Hippocampal gene expression profiling across 8 inbred strains: towards understanding the molecular basis of behaviour |
|
