|
| Status |
Public on Apr 30, 2015 |
| Title |
T-ALL_0370 |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow mononuclear cells obtained from patient with newly diagnosed T-ALL
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Bone marrow mononuclear cells
notch1 mutational status: Mutant
pten mutational status: Wild type
il7r mutational status: Wild type
age (years): 3.5
gender: Female
wbc at diagnosis (cells/mm3): 20200
induction failure: Remission
relapse: No Relapse
|
| Treatment protocol |
Five million primary T-ALL cells were preserved in 150 µL of thiocyanate solution at -70ºC until use.
|
| Growth protocol |
Mononuclear cells were ficoll-isolated from diagnostic bone marrow samples of T-ALL patients.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with the Illustra RNAspin Mini RNA Isolation Kit (GE Healthcare, Little Chalfont, UK), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were processed with the WT Expression Kit (Ambion, Inc., Austin, TX, USA) and GeneChip WT Terminal Labeling and Controls Kit (Affymetrix, Inc., Santa Clara, CA, USA), according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Samples were hybridized on the GeneChip Human Gene 1.0 ST Arrays (Affymetrix, Inc.), according to the manufacturer's instructions. Arrays were washed and stained with the GeneChip Fluidics Station 450.
|
| Scan protocol |
Arrays were scanned with the GeneChip Scanner 3000, according to the manufacturer's instructions.
|
| Description |
Diagnostic T-ALL sample
|
| Data processing |
Arrays were normalized and expression values were obtained with the default transcript level iterPLIER algorithm of the Affymetrix Expression Console software.
|
| |
|
| Submission date |
Sep 18, 2013 |
| Last update date |
May 01, 2015 |
| Contact name |
Andre Bortolini Silveira |
| E-mail(s) |
[email protected]
|
| Organization name |
Institut Curie
|
| Department |
U830
|
| Lab |
DRUM team
|
| Street address |
26 rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE50999 |
Gene expression data of diagnostic childhood T-ALL samples |
| GSE51001 |
PI3K inhibition synergizes with glucocorticoids but antagonizes with methotrexate in T-cell acute lymphoblastic leukemia. |
|
