|
| Status |
Public on Sep 18, 2013 |
| Title |
BJ_Y701F STAT1_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
BJ fibroblasts, Y701F STAT1 transfected
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: normal fibroblasts
treatment: Y701F STAT1
|
| Treatment protocol |
Cells were treated with 3 IU/ml of IFN beta for 6 hrs. Cells were transfected with empty lentiviral vector or vector containing Y701F STAT1 cDNA.
|
| Growth protocol |
Cells were cultured in DMEM supplemented with 5% FBS, 100 U/ml penicillin, and 100 g/ml streptomycin. Lentiviral vector transfected cells were selected by puromycin treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Nanodrop.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
rep2
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Sep 17, 2013 |
| Last update date |
Sep 18, 2013 |
| Contact name |
HyeonJoo Cheon |
| E-mail(s) |
[email protected]
|
| Phone |
216-445-9769
|
| Organization name |
Cleveland Clinic
|
| Department |
Molecular Genetics
|
| Lab |
NE2-252
|
| Street address |
9500 Euclid Ave
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44122 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (1) |
| GSE50954 |
Genes induced by IFN-beta, IFN-gamma or unphosphoraylted STAT1 in human fibroblasts |
|
