|
| Status |
Public on Nov 13, 2013 |
| Title |
Mepenzolate treatment, 25 hours (replication 2) |
| Sample type |
RNA |
| |
|
| Source name |
Lung tissue
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Mepenzolate
time: 25
|
| Treatment protocol |
For the intratracheal administration of mepenzolate, mice were maintained under anesthesia with chloral hydrate (500 mg/kg) and mepenzolate in PBS was administered via micropipette.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissue specimens using RNeasy kit (Qiagen, Hilden, Germany). Integrity of obtained RNA was assessed using Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA).
|
| Label |
biotin
|
| Label protocol |
The cDNA was prepared using GeneChip WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization and signal detection of Mouse Gene 1.0 ST Arrays (Affymetrix) was performed following the manufacturer's instruction using the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression profile of lung tissue
|
| Data processing |
The data was normalized by robust multichip analysis (RMA) using R/Bioconductor.
|
| |
|
| Submission date |
Sep 12, 2013 |
| Last update date |
Nov 13, 2013 |
| Contact name |
Kaoru Mogushi |
| E-mail(s) |
[email protected]
|
| Phone |
+81-3-5802-1797
|
| Organization name |
Juntendo University
|
| Department |
Intractable Disease Research Center
|
| Street address |
2-1-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8421 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE50836 |
Mepenzolate bromide displays beneficial effects in a mouse model of chronic obstructive pulmonary disease |
|
