|
| Status |
Public on Dec 10, 2013 |
| Title |
Gudjohnsson_004_7450_PP |
| Sample type |
RNA |
| |
|
| Source name |
skin
|
| Organism |
Homo sapiens |
| Characteristics |
patient: 7450
phenotype: lesional
tissue: lesional skin biopsy
|
| Treatment protocol |
At baseline, two biopsies were taken from each patient; one 6mm punch biopsy was obtained from lesional skin of each patient (involved sample) and the other from non-lesional skin (uninvolved sample). Non-lesional biopsies were taken from the buttocks or upper thighs. Biopsies were snap-frozen in liquid nitrogen, stored at -80°C. At processing the biopsy samples were homogenized while still frozen and total RNA isolation was performed using RNeasy kit protocol (Qiagen, Chatsworth, Ca, USA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAGEN RNEasy mini kits
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133 Human Genome Plus 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray TM Scanner 3000.
|
| Description |
lesional skin biopsy
|
| Data processing |
Robust Multichip Average (RMA)
|
| |
|
| Submission date |
Sep 11, 2013 |
| Last update date |
Dec 10, 2013 |
| Contact name |
William R Swindell |
| E-mail(s) |
[email protected]
|
| Organization name |
Harvard University
|
| Department |
Genetics
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE50790 |
Gene expression in psoriasis lesions and uninvolved skin |
|
