Primary HUVEC cultured on gelatin coated flasks derived from 7 different umbilical cords, isolated as described by Jaffe, et al. Cells were grown to one day post-confluence before stimulating with LPS or vehicle for 2 hours.
Treatment protocol
Control cells received an equal amount of HBSS as a vehicle control for LPS.
Extracted molecule
total RNA
Label
alpha-33P dCTP
Description
These samples were collected as part of a polysome profiling experiment in which monosome and polysome associated mRNA was collected via centrifugation and fractionation using an Isco fractionator. This sample is the control monosome fraction used to calculate a translation index as follows: (histamine polysome/histamine monosome)/(control polysome/control monosome).
Data processing
The value column was generated by dividing the SIGNAL_RAW values by the median signal intensity of the entire membrane. This was done in order to compare multiple array values.
