|
| Status |
Public on Feb 17, 2015 |
| Title |
Sort-purified Flvcr+/+ DP thymocytes replicate#1 |
| Sample type |
RNA |
| |
|
| Source name |
thymus
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Flvcr+/+
cell type: CD4+CD8+ DP thymocyte
strain: C57BL/6 background
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After FACS-sorting , DP thymocytes were washed twice with cold PBS and cell pellets were frozen and stored at -80C until all samples were collected. RNA was isolated using Qiagen RNeasy Plus Mini Kit per the manufacturer’s instructions and the yield was determined on a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). Samples that had an appropriate yield (typically 100ng total RNA) were subsequently analyzed for RNA integrity using an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
CD4+CD8+ thymocytes were isolated from thymuses of Flvcr+/+ mice and FACS sorted
WT1
|
| Data processing |
Signal intensities were quantile normalized using the Bioconductor package lumi
|
| |
|
| Submission date |
Aug 26, 2013 |
| Last update date |
Feb 17, 2015 |
| Contact name |
Mary Philip |
| E-mail(s) |
[email protected]
|
| Organization name |
Vanderbilt University Medical Center
|
| Department |
Medicine
|
| Street address |
777 PRB, 2220 Pierce Ave
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL17543 |
| Series (1) |
| GSE50202 |
Gene expression profile of murine Flvcr-deleted and control CD4+CD8+ double-positive (DP) thymocytes |
|
