|
| Status |
Public on Dec 31, 2013 |
| Title |
WT#3 |
| Sample type |
RNA |
| |
|
| Source name |
WT mouse embryo #3, E16.5
|
| Organism |
Mus musculus |
| Characteristics |
age: embryonic day 16.5
genotype/variation: wild type
|
| Treatment protocol |
No special treatment. After disecting, forebrains were directly subjected to RNA purification.
|
| Growth protocol |
Tbr mutant mice were maintained by backcross heterozygous Tbr1+/- mice with WT B6 mice for more than 10 generations. For experiment, Tbr1+/- female mated with Tbr1+/- male to generate all of +/+, +/- and -/- embryos. Only +/+ and -/- embryos were compared.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolated from freshly dissected E16.5 mouse forebrains (4 WT and 4 Tbr1-/-) and purified by TRIzol reagent .
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols for Mouse Genome 430 2.0 Array Following fragmentation, 15 ug of cRNA were hybridized for 17 hr at 45C on Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned witj Affymetrix GeneChip Scanner 3000 using the standard Affymetrix protocol,
|
| Description |
None
|
| Data processing |
the initial DAT files were processed by Affymetrix GeneChip Operating Software (GCOS) to generate the raw signal intensity CEL files.
|
| |
|
| Submission date |
Jul 25, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Yi-Ping Hsueh |
| E-mail(s) |
[email protected]
|
| Phone |
886-2-27899311
|
| Organization name |
Academia Sinica
|
| Department |
Institute of Molecular Biology
|
| Lab |
N514
|
| Street address |
128 Academia Road, Section 2, Nankang
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE49237 |
Analysis of TBR1 downnstream target genes in embryonic forebrains |
|
