|
| Status |
Public on Oct 01, 2013 |
| Title |
Embryonic ventral forebrain sample 2 |
| Sample type |
RNA |
| |
|
| Source name |
wild type E14.5 ventral forebrain
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD-1
developmental stage: embryo (E14.5)
tissue: ventral forebrain
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Secondary neurospheres derived from adult and embryonic brains and spinal cord
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (QIAGEN) was used for isolation and purification of total RNAs. The concentration of RNA was measured using RNA Assay Nanoprep Chip 6000 and Bioanalyzer (Agilent). To compare the expression profiles across the samples, Affymetrix GeneChip Mouse Genome 430 2.0 Array Platform.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the Terminal Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials • Heat cocktails at 99° for 5 minutes, then 42° for 5 minutes centrifuge at max speed for 1 minute (N.B. this deviates from Affy SOP). • Transfer 200μl of hyb solution to each array, then tape holes and parafilm. • Hybridize 16 hours at 45° at 60rpm• Fluidics washing is FS450_0001
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
Embryonic ventral forebrain sample 2
|
| Data processing |
Data were analyzed using Silicon Genetics GeneSpring Software GX7.3.1.
|
| |
|
| Submission date |
Jul 25, 2013 |
| Last update date |
Oct 01, 2013 |
| Contact name |
Masato Nakafuku |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Developmental Biology
|
| Street address |
3333 Burnet Avenue
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE49194 |
Expression data from neurospheres derived from the neocortex, striatum and subventricular zones of the adult mouse brain |
|
