Ryegrass tissue Perennial ryegrass (Lolium perenne L.) cv. Bronsyn was used throughout this study. Field grown samples, comprising mainly of viable leaves and pseudostems, were collected at mid-day from livestock-active monocultural paddocks at Dexcel, Hamilton, New Zealand during the peak of each season and frozen immediately in liquid nitrogen. Samples were transported in dry-ice and stored at -80°C until used. During spring and summer, care was taken so as to not to include any floral stems in the tissue sample. Grass samples were collected from pre-grazed (15-60 days post grazing) and post-grazed (1 day post grazing) ryegrass swards, which were visibly healthy and free of any pests.
Extracted molecule
total RNA
Extraction protocol
Construction of SAGE™ libraries RNA was extracted using TRIZOL® reagent (Invitrogen, CA, USA). For each SAGE™ library 100 µg each of pre- and post-grazed sample-derived of total RNA was were used and the libraries were created using I-SAGE™ or I-SAGE™ Long kit (Invitrogen, CA, USA) according to the manufacturer’s protocol. Pre- and post-grazed SAGE™ libraries were sequenced at the Australian Genome Research Facility (Brisbane, Australia) and the tags were extracted using the SAGE2000 software and combined to produce the transcript profile for the season
Description
TITLE: Transcriptome analysis reveals season-specific RbcS gene expression profiles in diploid perennial ryegrass (Lolium perenne L.) AUTHORS: Puthigae Sathish, Nimali Withana, Margaret Biswas, Catherine Bryant, Kerry Templeton, Muhannad Al-Wahb, Claudia Smith-Espinoza, John R. Roche, Kieran M. Elborough and Jonathan R. Phillips JOURNAL: Plant Biotechnology Journal, in press YEAR: 2007 http://www.blackwell-synergy.com/doi/abs/10.1111/j.1467-7652.2006.00228.x
