|
| Status |
Public on Jul 03, 2013 |
| Title |
day1-500ppm-2 |
| Sample type |
RNA |
| |
|
| Source name |
liver
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fischer (F344)
age: eight-weeks old
gender: male
tissue: Liver
phenobarbital dose (ppm): 500
timepoint: 1
|
| Treatment protocol |
Animals were dietary treated with 0, 50, 500 or 1000 ppm Phenobarbital
|
| Growth protocol |
Eight-week old male Fischer (F344) rats were obtained from Harlac Olac (Netherlands) and left to acclimatise for seven days prior to the commencement of the experiment. The animals were examined to ensure that they were physically and behaviourally normal. During the study the rats were kept at five animals per cage and under controlled lighting (12h light cycles), humidity (30-70%), air flow (15 changes per hour), and temperature (22±3 C). The rats were given access to mains water and powdered diet ad libitum. Animals were randomly assigned to cages and treatment groups based on their pre-experimental weight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 100mg of frozen liver sample using Trizol. The extracted RNA was evaluated as suitable for further analysis in it had an 260:280 ratio above two and a RIN number, as determined with on an Agilent bioanalyser, above eight.
|
| Label |
Cy3
|
| Label protocol |
100ng total RNA was labelled using the Agilent complete labelling and hybridisation kit
|
| |
|
| Hybridization protocol |
Labelled RNA was hybridized to microarray slides using the Agilent complete labelling and hybridisation kit
|
| Scan protocol |
The slides were scanned using an Agilent scanner (G2505B) and the data were extracted using the Agilent Feature Extraction v.10.1 software.
|
| Description |
Hepatic miRNA profile
|
| Data processing |
For each miRNA the raw data were threshold to 1, log2 transformed, and normalized to the 75th percentile. MiRNAs which were not flagged as present in at least 2 samples in any group were filtered out from further analysis
|
| |
|
| Submission date |
Jul 02, 2013 |
| Last update date |
Jul 03, 2013 |
| Contact name |
Nigel J Gooderham |
| E-mail(s) |
[email protected]
|
| Organization name |
Imperial College London
|
| Department |
Biomolecular Medicine
|
| Street address |
South Kensington Campus
|
| City |
London |
| ZIP/Postal code |
SW72AZ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL14889 |
| Series (1) |
| GSE48489 |
Dose and temporal effects of Phenobarbital on the liver miRNAome of male Fischer rats |
|
