|
| Status |
Public on Sep 10, 2015 |
| Title |
hST2-U3C 0h_rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
hST2-U3C 0h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: hST2-U3C
genotype/variation: STAT1 KO - hSTAT2 stable
agent: untreated
time: 0h
|
| Treatment protocol |
In vitro grwoing cells were starved over night in DMEM 4,5g Glucose/L with 2% FBS and Pen-Strep afterwards human IFNα (Millipore) has been added in 200U/ml for: 0h, 4h, 8h, 24h
|
| Growth protocol |
2fTGH and hST2-U3C cells were keept in DMEM 4,5g Glucose/L with 10% FBS and Pen-Strep. Cells were splited when they reached 80% - 90%.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using GeneMATRIX Universal RNA Purification Kit (EurX). Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays (LifeTechnologies)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 15
6898333051_L
replicate 3
|
| Data processing |
Data were background subtracted and quantile normalised in GenomeStudio (Illumina)
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
Sep 10, 2015 |
| Contact name |
Hans Bluyssen |
| E-mail(s) |
[email protected]
|
| Organization name |
Faculty of Biology, University of Adam Mickiewicz
|
| Department |
Department of Human Molecular Genetics
|
| Street address |
Umultowska 89
|
| City |
Poznań |
| ZIP/Postal code |
61-614 |
| Country |
Poland |
| |
|
| Platform ID |
GPL10904 |
| Series (2) |
| GSE48313 |
STAT2 and IRF9-dependent IFN-I signaling restores ISRE-mediated transcription and anti-viral activity independent of STAT1 (human) |
| GSE50007 |
STAT2 and IRF9-dependent IFN-I signaling restores ISRE-mediated transcription and anti-viral activity independent of STAT1 |
|
