|
| Status |
Public on Jun 27, 2013 |
| Title |
testes Nxt1 rep2 |
| Sample type |
RNA |
| |
|
| Source name |
adult testes_Nxt1 mutant individuals
|
| Organism |
Drosophila melanogaster |
| Characteristics |
genotype/variation: w; Nxt1[z2-0488]/Nxt1[DG05102]
tissue: adult testis
culture temperature: 25°C
|
| Treatment protocol |
Testes were manually dissected in testis buffer, snap frozen in liquid nitrogen, and stored at -80C until sufficient sample had been collected. Samples were processed into 350ul lysis buffer (RLT+b-ME) then shipped on dry ice to the microarray facility in Glasgow university.
|
| Growth protocol |
Flies were grown on standard cornmeal yeast sucrose agar medium, and collected for dissection within one day of eclosion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA easy (Qiagen) extraction of total RNA according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridisation was perfomed in GeneChip Hybridisation Oven 640, and washed with wash with GeneChip Fluidic Station 400
|
| Scan protocol |
scanning and quality control with GeneChip Scanner 3000 7G
|
| Description |
gene expression from Nxt1 mutant testes
|
| Data processing |
raw data were generated with GCOS/AGCC software. Data were normalised with an RMA normalisation across replicates
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
Jun 27, 2013 |
| Contact name |
Helen White-Cooper |
| E-mail(s) |
[email protected]
|
| Organization name |
Cardiff University
|
| Department |
Biosciences
|
| Street address |
Museum Avenue
|
| City |
Cardiff |
| ZIP/Postal code |
CF10 3AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE48312 |
Comparison of gene expression in wild type Drosophila testes with various meiotic arrest mutants |
|
