|
| Status |
Public on Dec 01, 2013 |
| Title |
Inclusion body myositis, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
MHC-I-positive microdissected myofibers
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: skeletal muscle
disease status: inclusion body myositis
|
| Treatment protocol |
Immuno-guided microdissection was performed on muscle biopsies in order to isolate MHC-I-positive myofibers.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction using Rneasy Micro kit(Qigen). Total RNA from each sample was amplified for ssDNA generation using the Ovation™ Pico WTA System (NuGEN Technologies, San Carlos, CA)
|
| Label |
biotin
|
| Label protocol |
ssDNA was fragmented and labelled with the Encore® Biotin Module (NuGEN Technologies)
|
| |
|
| Hybridization protocol |
According to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000.
|
| Description |
Samples were amplified using NugEn ovation WTA
|
| Data processing |
Chips were processed on an Affymetrix GeneChip Fluidics Station 450 using Affymetrix default analysis settings and global scaling as normalization method
|
| |
|
| Submission date |
Jun 25, 2013 |
| Last update date |
Dec 01, 2013 |
| Contact name |
Xavier Suárez-Calvet |
| Organization name |
Institut de Recerca Sant Pau
|
| Department |
Neurology
|
| Lab |
Neuromuscular
|
| Street address |
Sant Antoni Maria Claret, 167
|
| City |
Barcelona |
| ZIP/Postal code |
08025 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE48280 |
Expression data from inflammatory myopathies |
|
