|
| Status |
Public on Jun 28, 2013 |
| Title |
UnInfected-2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse lungs-uninfected
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Lung
strain: B6D2F1
infection: uninfected
time: control
|
| Treatment protocol |
PZA, one of the first line anti-TB drugs, was used to treat one group of Mtb infected mice. The compound was used at 150mg/kg body weight, dissolved in distilled water and administered through gavage five days a weeks. Treatment was started at 28 days post infection and continued up to 63 days post infection.
|
| Growth protocol |
B6D2F1 mice were infected with Mtb CDC1551 at ~2log10 (on day 0). At28 days post infection, one group of infected mice were treated with Pyrazinamide at 150mg/kg. Lung tissue from Uninfected, Untreated and PZA treated mice were isolated at Day 0, 28, 42 and 63 days post infection and used for total RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from the mouse lungs were isolated using TRIzol reagent (Invitrogen, CA, USA) and purified using RNA mini prep columns (Qiagen, CA, USA) according to the instructions of the manufacturers
|
| Label |
biotin
|
| Label protocol |
Total RNA samples were reverse transcribed and labled with biotin as suggested by the array manufacturer (affymetrix)
|
| |
|
| Hybridization protocol |
standard protocol used can be found at <http://www.cag.icph.org> from the center for advanced genomics of the Public Health Research Institute of UMDNJ was used for all the experiments
|
| Scan protocol |
standard protocol used can be found at <http://www.cag.icph.org> from the center for advanced genomics of the Public Health Research Institute of UMDNJ was used for all the experiments
|
| Description |
Gene expression data from uninfected mouse lungs
|
| Data processing |
Data from the scanned images were extracted using the software from Affymetrix and processed using PARTEK Genomics Suite for further analysis
|
| |
|
| Submission date |
Jun 17, 2013 |
| Last update date |
Jun 28, 2013 |
| Contact name |
Selvakumar Subbian |
| E-mail(s) |
[email protected]
|
| Phone |
973-854-3226
|
| Organization name |
PHRI, NJMS, Rutgers the State University of NJ
|
| Department |
Medicine
|
| Lab |
Laboratory of Mycobacterial Immunity and Pathogenesis
|
| Street address |
225 Warren Street
|
| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07103 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE48027 |
Host directed activity of Pyrazinamide in Mycobacterium tuberculosis infection |
|
