|
| Status |
Public on Jun 18, 2013 |
| Title |
Female Ctr |
| Sample type |
RNA |
| |
|
| Source name |
Liver from 3-week-old offspring mouse fed by CTR diet
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
strain: ICR
tissue: Liver
treatment: CTR diet
|
| Treatment protocol |
The female mice (5 weeks of age) were fed a non-purified commercial normal chow (Labo MR Stock; 2.31 kcal⁄g, 15.2% fat, 32.6% protein, 52.2% carbohydrate by energy; Nosan Co., Yokohama, Kanagawa, Japan) in the CTR group, or high-fat chow (CLEA Rodent Diet Quick Fat; 4.16 kcal⁄g, 31.2% fat, 23.9% protein, 44.9% carbohydrate by energy; CLEA Japan) in the HFD group, for 3−4 weeks. The female mice (8−9 weeks of age) were mated with male C57BL/6J mice (10-11 weeks of age) fed normal chow. Mothers continued on the same diet until pups reached 21 days of age, but some CTR and HFD female mice were fed high-fat and normal chow from gestational day 0 to produce HFD-PP (high-fat diet only during pre-pregnancy) and CTR-PP (high-fat diet only during gestation and lactation) groups, respectively. Offspring mice were housed with each dam for suckling.
|
| Growth protocol |
Female and male C57BL/6J mice were purchased from CLEA Japan, Inc. (Tokyo, Japan) and acclimated to our animal room (The Center for Environmental Health Science for the Next Generation, Research Institute for Science and Technology, Tokyo University of Science) for 7 days. All animals were treated and handled in accordance with national guidelines for the care and use of laboratory animals and with the approval of institutional animal care and use committee.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver tissues for gene expression analysis were homogenized in Isogen (Nippon Gene Co., Ltd., Tokyo, Japan). Total RNA was isolated according to the manufacturer's protocol and suspended in RNase-free water.
|
| Label |
Cy3
|
| Label protocol |
After purification of RNA by ethanol precipitation and RNeasy Micro Kit (Qiagen, Hilden, Germany), the integrity of extracted RNA was evaluated with capillary electrophoresis using a Bioanalyzer 2100 (Agilent Technologies, Inc., Santa Clara, CA, USA). Total RNAs were pooled for each group. Each of the pooled RNA samples was labeled by Cy3.
|
| |
|
| Hybridization protocol |
Cy3-labelled RNA was hybridized to a SurePrint G3 Mouse GE 8x60K microarray according to the protocol of Takara Bio, Inc. (Shiga, Japan).
|
| Scan protocol |
The microarray was washed using Gene Expression Wash Buffers Pack (Agilent Technologies) and scanned by a DNA microarray Scanner G2565CA (Agilent Technologies).
|
| Description |
Scanned by a DNA microarray Scanner G2565CA (Agilent Technologies) and obtained output images were normalized and digitalized by Agilent Feature Extraction software according to the Minimum Information About a Microarray Experiment (MIAME) guidelines and a pre-processing method for Agilent data.
|
| Data processing |
Scanner output images were normalized and digitalized by Agilent Feature Extraction software according to the Minimum Information About a Microarray Experiment (MIAME) guidelines and a pre-processing method for Agilent data.
|
| |
|
| Submission date |
Jun 17, 2013 |
| Last update date |
Jun 18, 2013 |
| Contact name |
Masakazu Umezawa |
| E-mail(s) |
[email protected]
|
| Phone |
+81-4-7124-1501
|
| Organization name |
Tokyo University of Science
|
| Department |
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences
|
| Lab |
Takeda Lab.
|
| Street address |
2641 Yamazaki
|
| City |
Noda |
| State/province |
Chiba |
| ZIP/Postal code |
278-8510 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13912 |
| Series (1) |
| GSE48014 |
Gene expression in the liver, effect of maternal high-fat diet during or prior to pregnancy |
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