|
| Status |
Public on Jun 13, 2007 |
| Title |
Creatine-treated, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus brain hemisphere
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C3HeB/FeJ
Age: 10-12 weeks at the beginning of the feeding regimen
|
| Treatment protocol |
6 mice were fed ad libitum on 1% creatine supplemented pellets for 6 months and 7 mice fed on equicaloric pellets without creatine (Altromin)
|
| Growth protocol |
Mice were bred and kept at the GSF animal facilities in isolated ventilated cages type II with wood shavings (Altromin, Lage, Germany) as bedding at a temperature of 20 to 24o C, humidity of 50 to 60%, 20 air exchanges per hour and a 12/12-hour light/dark cycle. They were tested for microorganisms in 12-week intervals according to the FELASA Guidelines to the level required for rederived mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy kit (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scaner 3000.
|
| Description |
Gene expression data from male mice.
|
| Data processing |
Genewise tests for significantly regulated transcripts between creatine treated and untreated mice were performed in the statistical programming language R with BioConductor using the dChip and the Robust Multichip Average (RMA) probeset summaries. Data are log-transformed and normalized with a lowess-smoother operating on MA-scale against the mean of the creatine treated group. Transcripts are called significantly regulated if the corresponding p-value of the Welch's t-test is below the 5% FDR significance level.
|
| |
|
| Submission date |
Jun 21, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
[email protected]
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5140 |
Creatine increases health and life span in mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
