|
| Status |
Public on Nov 21, 2013 |
| Title |
egl3 mutant, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
root epidermal cells from egl3 mutant
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype/variation: egl3-1 WER::GFP
tissue: root epidermal cells
|
| Treatment protocol |
Root were cut approximately 2 mm from the tip, including the entire developmental region.
|
| Growth protocol |
Seedlings were grown for 5 days in continuous light at 23C on vertically-oriented dishes containing MS media + 1% sucrose.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
WER::GFP-expressing cells were collected by FACS. Total RNA was extracted from GFP-positive cells using the Rneasy kit (Qiagen, Inc).
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was synthesized according to the standard Affymetrix protocol from poly(A) RNA present in the total RNA prep.
|
| |
|
| Hybridization protocol |
The cRNA was fragmented in the presence of heat and Mg2+, followed by hybridization to the Affymetrix ATH1 Arabidopsis genechip for 16 hr at 45C. The arrays were then washed and stained using the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Description |
m-EGL3_92
Analysis conducted at the University of Michigan
|
| Data processing |
The data were preprocessed in R using RMA with custom CDF files (http://brainarray.mbni.med.umich.edu/Brainarray/Database/CustomCDF/genomic_curated_CDF.asp).
|
| |
|
| Submission date |
Jun 04, 2013 |
| Last update date |
Nov 22, 2013 |
| Contact name |
John Schiefelbein |
| E-mail(s) |
[email protected]
|
| Phone |
734-764-3580
|
| Organization name |
University of Michigan
|
| Department |
Molecular, Cellular, and Developmental Biology
|
| Street address |
830 North University Avenue
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL13838 |
| Series (1) |
| GSE47632 |
Expression data from the Arabidopsis root epidermis mutants |
|
