|
| Status |
Public on Nov 01, 2015 |
| Title |
Allo-960S7, biological rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
leaf of newly synthesized wheat Allo-960 at selfed generations 7
|
| Organism |
Triticum aestivum |
| Characteristics |
tissue: leaf
|
| Growth protocol |
All plants were grown in chambers under controlled conditions at 24/20°C day/night of 16 h day-length.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Followed Expression Analysis Technical Manual, 2001, Affymetrix.
|
| Scan protocol |
Followed Expression Analysis Technical Manual, 2001, Affymetrix.
|
| Description |
Allo-960S7-2
Gene expression data from leaf of newly synthesized wheat Allo-960 at selfed generations 7
|
| Data processing |
The raw CEL data were normalized with the Robust Multichip Average (RMA) method using the R software 'limma' package. Genes that were differentially expressed among genotypes were identified by performing a t-moderated test ebayes (P value<0.05).
|
| |
|
| Submission date |
Jun 03, 2013 |
| Last update date |
Nov 01, 2015 |
| Contact name |
bao qi |
| E-mail(s) |
[email protected]
|
| Organization name |
northeast normal university
|
| Street address |
5268, renmindajie
|
| City |
changchun |
| State/province |
jilin province |
| ZIP/Postal code |
130024 |
| Country |
China |
| |
|
| Platform ID |
GPL3802 |
| Series (1) |
| GSE47617 |
Transcriptome modification to the BBAA subgenomes of common wheat since allohexaploidization is largely irreversible |
|
