|
| Status |
Public on Dec 02, 2013 |
| Title |
Activated_Tcells_PsoriasisPatient3 |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs
|
| Organism |
Homo sapiens |
| Characteristics |
group: Psoriasis Patient
|
| Treatment protocol |
anti-CD3 and anti-CD28 in vitro stimulation for 72 hours
|
| Growth protocol |
Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of patients and controls by centrifugation on a Ficoll-Paque PLUS (GE Healthcare Biosciences AB, Uppsala, Sweden) density gradient. PBMCs were then seeded in complete RPMI 1640 medium supplemented with 100 U/ml penicillin, 100 U/ml streptomycin, 10% fetal bovine serum, 2 mM L-glutamine and 50 μM mercaptoethanol, and activated with 1μg/ml of anti-CD3 and anti-CD28 antibodies (eBioscience, San Diego, CA, USA). After 72 hours of stimulation, activated T cells were harvested by centrifugation and processed for flow cytometry study or frozen at -20ºC for subsequent RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy mini kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Standard Illumina labeling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Patient 3 sample
|
| Data processing |
log2 transformation and quantile normalization
|
| |
|
| Submission date |
Jun 03, 2013 |
| Last update date |
Dec 02, 2013 |
| Contact name |
Antonio Julià |
| E-mail(s) |
[email protected]
|
| Phone |
+34 934029082
|
| Organization name |
Vall d'Hebron Hospital Research Institute
|
| Department |
Rheumatology Research Group
|
| Lab |
Rheumatology Research Group
|
| Street address |
c/ Baldiri Reixac nº15
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE47598 |
Activated T cell gene expression profile associated with Psoriasis |
|
