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Sample GSM115180 Query DataSets for GSM115180
Status Public on Aug 30, 2006
Title Diffuse large B-cell lymphoma VUMC_L53_b32_s40
Sample type genomic
 
Channel 1
Source name lymph node
Organism Homo sapiens
Characteristics Diffuse large B-cell lymphoma
Gender: female
Biomaterial provider VUMC, Amsterdam, The Netherlands
Extracted molecule genomic DNA
Extraction protocol DNA isolation protocol available via the VUMC MACF website
Also described in Van den IJssel et al, Nucleic Acids Res. 2005 Dec 16;33(22):e192
Label Cy3
Label protocol DNA labeling protocol available via the VUMC MACF website
Also described in Van den IJssel et al, Nucleic Acids Res. 2005 Dec 16;33(22):e192
 
Channel 2
Source name Male reference DNA
Organism Homo sapiens
Characteristics Pooled DNA from the blood of 10 normal individuals
Gender: male
Biomaterial provider VUMC, Amsterdam, The Netherlands
Extracted molecule genomic DNA
Extraction protocol DNAzol (Invitrogen) according to manufacturer's protocol
Label Cy5
Label protocol DNA labeling protocol available via the VUMC MACF website
Also described in Van den IJssel et al, Nucleic Acids Res. 2005 Dec 16;33(22):e192
 
 
Hybridization protocol Hybridization protocol available via the VUMC MACF website
Also described in Van den IJssel et al, Nucleic Acids Res. 2005 Dec 16;33(22):e192
Scan protocol Microarray Scanner G2505B (Agilent Technologies), default settings
Description protocols available via the VUMC MACF website
Data processing Using ImaGene as described in Carvalho et al (2004) J.Clin.Path., vol. 57, 644-646. Normalization was done to the mode value of the log2ratio's.
 
Submission date Jun 20, 2006
Last update date Aug 30, 2006
Contact name Daoud Sie
E-mail(s) [email protected]
Phone +31 20 4442428
Organization name Vrije Universiteit Medical Center
Department Pathology
Lab Microarray Core Facility
Street address De Boelelaan 1117
City Amsterdam
ZIP/Postal code 1081 HV
Country Netherlands
 
Platform ID GPL3443
Series (1)
GSE5051 Cross-Platform Array Comparative Genomic Hybridization (array CGH) Meta-Analysis

Data table header descriptions
ID_REF
Signal Median CH1 Spot signal in channel 1 (Cy3)
Background Median CH1 Background signal channel 1 (Cy3)
Signal Median CH2 Spot signal in channel 2 (Cy5)
Background Median CH2 Background signal channel 2 (Cy5)
Log2Ratio
Flag A flag to highlight spots that suffer from poor printing and other experimental artefacts (0 acceptable; 2 not acceptable)
VALUE Normalized value of the log2ratio, features with flag 2 are excluded

Data table
ID_REF Signal Median CH1 Background Median CH1 Signal Median CH2 Background Median CH2 Log2Ratio Flag VALUE
1 297.5 271 191 162 -0.130060541 3
2 341 265 1721 158 -4.36217455 0
3 524 264.5 210 156 2.264703226 3
4 1462 251 6732 160 -2.440133615 0
5 1672 258 7533 162.5 -2.381980372 0
6 1790 246 7629 156 -2.27501477 3
7 1217 222 6433 162 -2.655927089 0
8 1129.5 199 5779 147 -2.597569279 0
9 560 203 5917 144 -4.015325246 0
10 1068 194 3779 140 -2.057836866 0 0.879347082
11 1104 180 3852 139.5 -2.006426269 0 0.879347082
12 1038 163 3555.5 133 -1.967695619 0 0.879347082
13 315 164 196 137 1.35576169 0
14 215 171.5 636 135 -3.525723297 0
15 263 179 592 142 -2.421463768 0
16 1063.5 173 4450.5 144 -2.273828315 0 0.617526771
17 905 169 3743.5 140 -2.291621174 0 0.617526771
18 936 167 3807 144 -2.251970198 0 0.617526771
19 635.5 171 2989.5 148 -2.612902214 0
20 635 270 1826 163 -2.1878198 3

Total number of rows: 17328

Table truncated, full table size 717 Kbytes.




Supplementary data files not provided

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