|
| Status |
Public on Mar 30, 2014 |
| Title |
IL6raKO macrophages biological replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
IL6ralpha flox/flox LysMCre tg/wt mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone marrow
cell type: macrophage
genotype/variation: IL6raKO
agent: IL-6
|
| Treatment protocol |
Bone marrow-derived macrophages were serum-starved overnight and subsequently treated with 50ng/ml rec. murine IL-6 for 4 hours
|
| Growth protocol |
primary murine bone marrow cells were differentiated into macophages in the presence of 10ng/ml rec. murine M-CSF for 10 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from cells using the Qiagen RNeasy Kit (Qiagen); Quality of RNA was assessed using the ExperionTM RNA StdSens Analysis Kit (BIO-RAD)
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cDNA was synthesized using GeneChip Whole Transcript Sense Target Labeling Assay (Affymetrix)
|
| |
|
| Hybridization protocol |
cDNAs were hybridized for 17 h at 45°C to GeneChip Mouse Gene 1.0 ST Arrays (Affymetrix)
|
| Scan protocol |
Arrays were washed and stained in the GeneChip Fluidics Station 450 (Affymetrix) and scanned on a GeneChip Scanner 3000 7G (Affymetrix)
|
| Description |
IL-6 treated bone marrow-derived macrophages from IL6radmyel animals biological replicate 3
IL6raKO_3
|
| Data processing |
Affymetrix Powertools and robust multi-array average (RMA) were used for background correction, quantile normalization and summarization of the raw intensity values. RMA, statistical analyses (Student’s t-test), calculation of the fold change of intensity values and graphical illustrations were performed using R software (version 2.13.1) along with the Bioconductor software package. Genes were considered significantly deregulated if p-values ≤ 0.05.
probe group file: MoGene-1_0-st-v1.r4.pgf
meta-probeset file: MoGene-1_0-st-v1.r4.mps
|
| |
|
| Submission date |
May 28, 2013 |
| Last update date |
Mar 30, 2014 |
| Contact name |
Jan Mauer |
| E-mail(s) |
[email protected]
|
| Organization name |
BioMed X Innovation Center
|
| Street address |
Im Neuenheimer Feld, 583
|
| City |
Heidelberg |
| State/province |
BW |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE47426 |
Expression Data from murine control and IL6ralpha-deficient macrophages stimulated with Interleukin-6 |
|
