|
| Status |
Public on May 23, 2013 |
| Title |
MeCP2_3 |
| Sample type |
RNA |
| |
|
| Source name |
primary cortical neurons, MeCP2 shRNA
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57/Bl6J
days in vitro: 16
tissue: E16 embryonic cortex
treatment: MeCP2 shRNA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Five (5) ug of fragmented, biotin-labeled cDNA was hybridized to Affymetrix Mouse Genome 430 2.0 Gene Chips for 18 hours, then washed/stained according to manufacturer's recommendations using the Affymetrix 450 fluidics station.
|
| Scan protocol |
Gene chips were scanned using an Affymetrix 3000 array scanner.
|
| Description |
2 week shRNA treatment
|
| Data processing |
R/Bioconductor was used to apply the Robust Multi-Array Average (RMA) methodology to generate expression values for the combined samples.
|
| |
|
| Submission date |
May 21, 2013 |
| Last update date |
May 23, 2013 |
| Contact name |
Thomas A Lanz |
| E-mail(s) |
[email protected]
|
| Phone |
860-441-3551
|
| Organization name |
Pfizer
|
| Department |
Global Pathology and Investigative Toxicology
|
| Street address |
Eastern Point Rd MS# 8274-1224
|
| City |
Groton |
| State/province |
CT |
| ZIP/Postal code |
06340 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE47150 |
Microarray profiling of primary neurons transduced with shRNA for multiple ASD-implicated genes |
|
