|
| Status |
Public on Oct 31, 2013 |
| Title |
siCont |
| Sample type |
RNA |
| |
|
| Source name |
HCT116, siCont
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HCT116
sirna: siControl
|
| Treatment protocol |
H1299 cells were infected with adenoviruses expressing p53, p63g or p73b at 25 moi. HCT116 cells were transfected with siRNAs targeting AKR1B10 or negative control. 4h after the transfection, these cells were treated with adriamycin (0.5 ug/ml). 24h after the treatment, siRNAs were transfected into cells again.
|
| Growth protocol |
H1299 and HCT116 cells were cultured in DMEM and McCoy's 5A medium with 10% FCS, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
24h after infection or second transfection, total RNA was extracted by RNeasy mini kit (QIAGEN) according to manufacturer's protocol. RNA was quantified using the NanoDrop spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Description |
Gene expression after the transfection of siControl without adriamycin treatment
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
Log2 values were calculated by Limma (R package) for the normalization in each cell line.
|
| |
|
| Submission date |
May 20, 2013 |
| Last update date |
Oct 31, 2013 |
| Contact name |
Masashi Idogawa |
| Organization name |
Sapporo Medical University
|
| Street address |
S1W17
|
| City |
Sapporo |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL14550 |
| Series (1) |
| GSE47096 |
The effect on gene expression by p53 family members and the knockdown of AKR1B10 |
|
