|
| Status |
Public on Dec 31, 2006 |
| Title |
Untreated cells pc12 tmt contr_1_Signal |
| Sample type |
RNA |
| |
|
| Source name |
commercial cell line
|
| Organism |
Rattus norvegicus |
| Characteristics |
rat pheocromocitoma cell line
|
| Treatment protocol |
no treatment (cells treated with cell culture medium alone)
|
| Growth protocol |
cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rneasy mini kit extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip RAT 230A Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner 2500
|
| Description |
untreated cells pc12 tmt contr_1_Signal
|
| Data processing |
Raw data have been normalized using gc-RMA methods, followed by an additional “per gene normalisation” the data for a given gene is normalised to the median expression level of that gene across all samples.
|
| |
|
| Submission date |
Jun 13, 2006 |
| Last update date |
Jun 14, 2006 |
| Contact name |
camilla bernardini |
| E-mail(s) |
[email protected]
|
| Phone |
+390630154711
|
| Organization name |
UCSC
|
| Department |
Human Anatomy
|
| Street address |
L.go F.Vito 1
|
| City |
Rome |
| ZIP/Postal code |
00186 |
| Country |
Italy |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE5073 |
Effect of Trimethyltin on PC12 cells |
|
