|
| Status |
Public on Sep 05, 2013 |
| Title |
Myeloma cell line-U266-CD138- |
| Sample type |
RNA |
| |
|
| Source name |
Myeloma cell line-U266-CD138-
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: myeloma cell line U266
cell fraction: CD138-
|
| Treatment protocol |
Cells were incubated with CD138-microbeads for 20mins at 4 degree Celsius, and autoMACS and CD138 microbeads (Miltenyi Biotec Inc., Auburn, CA) were used for sorting.
|
| Growth protocol |
Cells were cultured in RPMI 1640 containing 2mM L-glutamine, 10% heat-inactivated fetal bovine serum (FBS), penicillin (100U/mL), and streptomycin (100 μg/mL) in a humidified incubator at 37°C in 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Microarray-based gene expression data from U266-CD138-
|
| Data processing |
affymetrix-algorithm-name = rma-gene-default
program-name = Expression Console
|
| |
|
| Submission date |
May 10, 2013 |
| Last update date |
Sep 05, 2013 |
| Contact name |
Fenghuang Zhan |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Iowa
|
| Street address |
285 Newton Rd, 3269A CBRB
|
| City |
IOWA CITY |
| State/province |
IA |
| ZIP/Postal code |
52242 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE46816 |
Gene expression data from 8 pairs of CD138+/- myeloma cell lines |
|
