|
| Status |
Public on Jul 06, 2006 |
| Title |
PGC-1alpha -/- differentiated brown fat cells expressing a siRNA specific for PGC-1beta knockdown (rep2) |
| Sample type |
RNA |
| |
|
| Source name |
brown fat cells differentiated for seven days
|
| Organism |
Mus musculus |
| Characteristics |
immortalized cells derived from brown fat tissue of PGC-1alpha KO mouse (mixed background), expressing a siRNA specific for PGC-1beta knockdown.
|
| Extracted molecule |
total RNA |
| Label |
biotinylation
|
| |
|
| Description |
Cells immortalized through SV40 driven largeT antigen expression
|
| Data processing |
Array normalization, expression value calculation and clustering analysis were performed using DNA-Chip Analyzer. The Invariant Set Normalization method was used to normalize arrays at probe cell level to make them comparable, and the model-based method was used for probe-selection and computing expression values.
|
| |
|
| Submission date |
Jun 08, 2006 |
| Last update date |
Jun 12, 2006 |
| Contact name |
Marc Uldry |
| E-mail(s) |
[email protected]
|
| Phone |
6176324661
|
| Organization name |
Dana Farber Cancer Institute
|
| Street address |
1 Jimmy Fund Way
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5042 |
Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation |
|
