|
| Status |
Public on Nov 24, 2014 |
| Title |
100031_PBMC_WNV |
| Sample type |
RNA |
| |
|
| Source name |
PBMC, severe disease, WNV
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral blood mononuclear cells (PBMCs)
treatment: West Nile virus (WNV)
age: 57
gender: male
batch: 1
phenotype: severe disease
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from PBMC and macrophage samples was extracted using the RNeasy mini kit (Qiagen, CA).
|
| Label |
biotin
|
| Label protocol |
Preparation of cDNA, cRNA, and labeling were carried out by the Yale Center for Genomic Analysis using the standard Illumina protocols.
|
| |
|
| Hybridization protocol |
Hybridization buffer from the BeadChip kit (Illumina) was mixed with 1500 ng of biotin-labeled cRNA, heated to 65°C for 5 minutes, and then loaded onto the BeadChip. The BeadChips were sealed in a hybridization chamber and placed in an oven at 58°C with a rocker for 16-20 hours. After the hybridization, the BeadChips were washed and stained in a series of washes and stains as outlined in the Illumina protocol.
|
| Scan protocol |
The BeadChips were scanned on the Illumina Iscan.
|
| Data processing |
The data were normalised using quantile normalisation with lumi in R.
|
| |
|
| Submission date |
May 06, 2013 |
| Last update date |
Nov 24, 2014 |
| Contact name |
Hailong Meng |
| Organization name |
Yale University
|
| Street address |
300 George Str
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06525 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE46681 |
Profiling the successful response to infection with West Nile virus |
|
