cell type: peripheral blood mononuclear cells (PBMCs) treatment: West Nile virus (WNV) age: 76 gender: male batch: 2 phenotype: asymptomatic
Extracted molecule
total RNA
Extraction protocol
Total RNA from PBMC and macrophage samples was extracted using the RNeasy mini kit (Qiagen, CA).
Label
biotin
Label protocol
Preparation of cDNA, cRNA, and labeling were carried out by the Yale Center for Genomic Analysis using the standard Illumina protocols.
Hybridization protocol
Hybridization buffer from the BeadChip kit (Illumina) was mixed with 1500 ng of biotin-labeled cRNA, heated to 65°C for 5 minutes, and then loaded onto the BeadChip. The BeadChips were sealed in a hybridization chamber and placed in an oven at 58°C with a rocker for 16-20 hours. After the hybridization, the BeadChips were washed and stained in a series of washes and stains as outlined in the Illumina protocol.
Scan protocol
The BeadChips were scanned on the Illumina Iscan.
Data processing
The data were normalised using quantile normalisation with lumi in R.
