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Sample GSM112970 Query DataSets for GSM112970
Status Public on Nov 03, 2006
Title mating responsive genes 18
Sample type RNA
 
Source name female lower reproductive tract
Organism Drosophila melanogaster
Characteristics Females from this sample were mated then dissected 6hrs postmating.
Treatment protocol Females were all same aged (3 days old) and either mated or not. Mated females were dissected at the following timeepoints: 0, 3, 6, 24 hrs postmating. Unmated (control) females were dissected at times interspersed with dissections of mated females.
Growth protocol 12 hr light/dark cycle 23C + or - 2C holding and culturing regimes
Extracted molecule total RNA
Extraction protocol TRIzol - chloroform
Label Biotin (Enzo RNA transcript Labeling Kit)
 
Description Three-day-old mated and unmated females were dissected to remove the lower reproductive tract (upper uterus, sperm-storage organs, and accessory glands). Mated females were dissected either immediately following mating (0 hr) or at 3, 6, or 24 hrs following the termination of mating. Tracts of 12-40 females of like category were pooled and total RNA extracted via a TRIzol-based protocol. Processing and labeling of transcript was performed by the Molecular Biology Core Facility at the Medical College of Georgia. Arrays from mated females at the different timepoints were compared to unmated females. This sample is also known as "565.49uga.cel" and "6hrUGA49.CEL". keywords: reproduction, reproductive tract, accessory gland proteins, sperm
Data processing RMA (Irizarry 2003), probe-based linear model (Chu et al 2002)
 
Submission date Jun 06, 2006
Last update date Nov 03, 2006
Contact name Paul Mack
E-mail(s) [email protected]
Phone 706 542-1578
Fax 706 542-3910
Organization name University of Georgia
Department Genetics
Lab Bender
Street address 1057 Green St.
City Athens
State/province GA
ZIP/Postal code 30602-7223
Country USA
 
Platform ID GPL72
Series (1)
GSE5012 mating response genes

Data table header descriptions
ID_REF
VALUE raw signal - measure of transcript abundance utilizing input from entire probeset for each gene
ABS_CALL Statistical estimation of presence (P), absence (A), or marginal (M) based on entire probeset input
DETECTION P-VALUE p-value indicating significance level of the detection call (note that p-values for "A" calls are > 0.06, "M" calls in range 0.04 - 0.06)

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
141200_at 23.5 A 0.413046
141201_at 16 A 0.801637
141202_at 68.2 A 0.070073
141203_at 45.3 A 0.062021
141204_at 276.8 P 0.003815
141205_at 11.4 A 0.48748
141206_at 625.1 P 0.000762
141207_at 1.8 A 0.834732
141208_at 188.3 P 0.001432
141209_at 446.5 P 0.015164
141210_at 16.4 A 0.48748
141211_at 100.2 P 0.03202
141212_at 721.1 P 0.000762
141213_at 41.1 A 0.062021
141214_at 1880.8 P 0.000491
141215_at 352.7 M 0.042142
141216_at 105 P 0.015164
141217_at 526.9 P 0.012921
141218_at 40.3 P 0.007827
141219_at 60 P 0.009283

Total number of rows: 14010

Table truncated, full table size 354 Kbytes.




Supplementary data files not provided

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